Effect of propolis supplementation on haematological and biochemical parameters in lambs

Authors

DOI:

https://doi.org/10.17159/sajas.v55i9.03

Keywords:

alternative therapy, antioxidant, blood parameters, propolis

Abstract

This study evaluated the effects of oral propolis supplementation on haematological and metabolic indicators and coagulation parameters in neonatal lambs. Forty healthy newborn lambs were randomly assigned to either a control group or a treatment group receiving 1.5 mL of propolis extract daily for 15 days. Blood samples were collected on days 0, 7, 21, and 45 to assess for changes over time. The results showed that propolis did not cause any harmful effects on liver or kidney function, and all blood parameters remained within normal physiological ranges. Although most changes in biochemical markers were time-related, lambs supplemented with propolis showed slight improvements in lipid metabolism, including numerically lower cholesterol and triglyceride levels at specific time points. Moreover, lambs in the propolis group tended to gain more weight over time, suggesting possible benefits for early growth. Minor differences were observed in some clotting parameters, but no adverse effects on blood coagulation were detected. These preliminary findings suggest that propolis may be considered a complementary strategy in lamb production, although further studies are needed to confirm its long-term benefits. Propolis-use may help facilitate a reduction in the use of synthetic additives in small ruminant production systems.

(Submitted 21 July 2025; Accepted 12 September 2025; Published 30 September 2025)

References

Abdlazez, S.T. & Saleh, H.H., 2016. Effect of different doses of propolis on growth performance, some carcass characteristics and lipid oxidation stability of Awassi lambs. Alexandria Journal of Veterinary Sciences, 9:62–71.

Al-Tamimi, A.M., 2020. Honey does not adversely impact blood lipids of adult men and women: A randomized cross-over trial. Nutrition Research, 74:87–95. DOI: https://doi.org/10.1016/j.nutres.2019.11.012

Aydogdu, U., Sen, I., & Guzelbektes, H., 2019. Buzağılarda pasif transfer yetmezliğinin belirlenmesinde kullanılan yöntemler. Manas Journal of Agriculture and Veterinary Sciences, 9(2):104–111.

Badawy, H.S., 2021. Effect of propolis as a feed additive on nutritional and productive performance of pregnant ewes and their lambs under Halaib-Shalateen pastures condition. Journal of Animal and Poultry Production, 12(1):19–26. DOI: https://doi.org/10.21608/jappmu.2021.149445

Bhadauria, M., 2012. Propolis prevents hepatorenal injury induced by chronic exposure to carbon tetrachloride. Evidence-Based Complementary and Alternative Medicine, 2012:1–12. DOI: https://doi.org/10.1155/2012/235358

Buratti, S., Benedetti, S., & Cosio, M.S., 2007. Evaluation of the antioxidant power of honey, propolis and royal jelly by different spectrophotometric and electrochemical methods. Food Chemistry, 104:1046–1053. DOI: https://doi.org/10.1016/j.talanta.2006.07.006

Castaldo, S. & Capasso, F., 2002. Propolis, an old remedy used in modern medicine. Fitoterapia, 73(1):S1–S6. DOI: https://doi.org/10.1016/S0367-326X(02)00185-5

Cécere, B.G., da Silva, A.S., Molosse, V.L., Alba, D.F., Leal, K.W., da Rosa, G., Souza, C.F., Baldissera, M.D., Campigotto, G., dos Santos, D.S., Santurio, J.M., Rampazzo, L., Glombowsky, P., Ourique, A.F., Stefani, L.M., Galli, G.M., Wagner, R., Mendes, R.E., Bottari, N.B., & Vedovatto, M., 2021. Addition of propolis to milk improves lactating lamb’s growth: effect on antimicrobial, antioxidant and immune responses in animals. Small Ruminant Research 194:106265. DOI: https://doi.org/10.1016/j.smallrumres.2020.106265

da Silva, F.G.B., Yamamoto, S.M., da Silva, E.M.S., Queiroz, M.A.A., Gordiano, L.A., & Formiga, M.A., 2015. Propolis extract and sodium monensin on ruminal fermentation and haematological parameters in sheep. Acta Scientiarum. Animal Sciences, 37(3):273–280. DOI: https://doi.org/10.4025/actascianimsci.v37i3.25725

de Melo Garcia, P.H., Guerra, R.R., de Oliveira, J.S., de Lima Júnior, D.M., de Almeida, V.V.S., da Silva, E.G., & Ribeiro, N.L., 2022. Red propolis extract in the diet of confined sheep: Morphometric alterations of the digestive system [Preprint]. DOI: https://doi.org/10.21203/rs.3.rs-1882436/v1

George, D. & Mallery, P., 2010. SPSS for Windows Step by Step: A Simple Guide and Reference, 10th edition. Pearson, Boston, USA.

Ghisalberti, E.L., 1979. Propolis: a review. Bee World 60(2):59–84. DOI: https://doi.org/10.1080/0005772X.1979.11097738

Grant, K.A., McLauchlin, J., & Amar, C., 2013. Infant botulism: advice on avoiding feeding honey to babies and other possible risk factors. Community Practitioner, 86(7):44–46.

Hallajzadeh, J., Milajerdi, A., Amirani, E., Attari, V.E., Maghsoudi, H., & Mirhashemi, S.M., 2021. Effects of propolis supplementation on glycaemic status, lipid profiles, inflammation and oxidative stress, liver enzymes, and body weight: A systematic review and meta-analysis of randomized controlled clinical trials. Journal of Diabetes and Metabolic Disorders, 20:831–843. DOI: https://doi.org/10.1007/s40200-020-00696-w

Hogan, I., Doherty, M., Fagan, J., Kennedy, E., Conneely, M., Brady, P., Ryan, C., & Lorenz, I., 2015. Comparison of rapid laboratory tests for failure of passive transfer in the bovine. Irish Veterinary Journal, 68(1):18. DOI: https://doi.org/10.1186/s13620-015-0047-0

Kolaylı, S., Sahin, H., Malkoc, M., Cakir, H., Kara, Y., Bektas, Ö., & Kaltalioglu, K., 2022. Does ethanolic propolis extract affect blood clotting parameters? Progress in Nutrition, 24(1):c2022028. DOI: https://doi.org/10.23751/pn.v24i1.12017

Koya-Miyata, S., Arai, N., Mizote, A., Taniguchi, Y., Ushio, S., Iwaki, K., & Shigeharu, F., 2009. Propolis prevents diet-induced hyperlipidaemia and mitigates weight gain in diet-induced obesity in mice. Biological and Pharmaceutical Bulletin, 32(12):2022–2028. DOI: https://doi.org/10.1248/bpb.32.2022

Linécio, M., Garcia, R.C., Galhardo, D., de Oliveira, N.T.E., & de Mello Peixoto, E.C.T., 2022. Extrato alcoólico de própolis no controle de verminoses em ovinos. Research, Society and Development, 11(1):e1711120617. DOI: https://doi.org/10.33448/rsd-v11i1.20617

Maden, M., Birdane, F.M., Altunok, V., & Dere, S., 2004. Serum and colostrum/milk alkaline phosphatase activities in the determination of passive transfer status in healthy lambs. Revue de Medecine Veterinaire, 155(11):565–569.

Ohkuara, N., Maruyama, K., & Kihara-Negishi, F., 2020. Possible antithrombotic properties of propolis. Journal of Applied Nutrition, 7(1):1–9. DOI: https://doi.org/10.5455/ja.20190807071847

Pérez, R., Burgos, V., Marín, V., Camins, A., Olloquequi, J., González-Chavarría, I., Ulrich, H., Wyneken, U., Luarte, A., Ortiz, L., & Paz, C., 2023. Caffeic acid phenethyl ester (CAPE): Biosynthesis, derivatives and formulations with neuroprotective activities. Antioxidants, 12(8):1500. DOI: https://doi.org/10.3390/antiox12081500

Salehi-Sahlabadi, A., Chhabra, M., Rahmani, J., Momeni, A., Karam, G., Nattagh-Eshtivani, E., Nouri, M., Clark, C., Salehi, P., & Hekmatdoost, A., 2020. The effect of propolis on anthropometric indices and lipid profile: A systematic review and meta-analysis of randomised controlled trials. Journal of Diabetes and Metabolic Disorders, 19:1835–1843. DOI: https://doi.org/10.1007/s40200-020-00604-2

Samadi, N., Mozaffari-Khosravi, H., Rahmanian, M., & Askarishahi, M., 2017. Effects of bee propolis supplementation on glycaemic control, lipid profile and insulin resistance indices in patients with type 2 diabetes: A randomised, double-blind clinical trial. Journal of Integrative Medicine, 15(2):124–134.

Silva, J.A., Ítavo, C.C.B.F., Ítavo, L.C.V., Graça Morais, M., Silva, P.C.G., Ferelli, K.L.S.M., & Souza Arco, T.F.F., 2019a. Dietary addition of crude form or ethanol extract of brown propolis as nutritional additive on behaviour, productive performance and carcass traits of lambs in feedlot. Journal of Animal and Feed Sciences, 28(1):31–40. DOI: https://doi.org/10.22358/jafs/105442/2019

Silva, J.B., Paiva, K.A.R., Costa, K.M.F.M., Viana, G.A., Araújo Júnior, H.N., Bezerra, L.S., Freitas, C.I.A., & Batista, J.S., 2019b. Hepatoprotective and antineoplastic potential of red propolis produced by the bees Apis mellifera in the semiarid of Rio Grande do Norte, Brazil. Pesquisa Veterinária Brasileira, 39(9):744–756. DOI: https://doi.org/10.1590/1678-5150-pvb-6214

Talas, Z.S. & Gulhan, M.F., 2009. Effects of various propolis concentrations on biochemical and haematological parameters of rainbow trout (Oncorhynchus mykiss). Ecotoxicology and Environmental Safety, 72(7):1994–1998. DOI: https://doi.org/10.1016/j.ecoenv.2009.04.011

Tsamesidis, I., Egwu, C.O., Samara, D., Vogiatzi, D., Lettas, A., & Lymperaki, E., 2022. Effects of Greek honey and propolis on oxidative stress and biochemical parameters in regular blood donors. Journal of Xenobiotics, 12(1):13–20. DOI: https://doi.org/10.3390/jox12010002

Zhang, Y.X., Yang, T.T., Xia, L., Zhang, W.F., Wang, J.F., & Wu, Y.P., 2017. Inhibitory effect of propolis on platelet aggregation in vitro. Journal of Healthcare Engineering, 2017:3050895. DOI: https://doi.org/10.1155/2017/3050895

Zhao, J.Q., Wen, Y.F., Bhadauria, M., Nirala, S.K., Sharma, A., Shrivastava, S., Shukla, S., Agrawal, O.P., & Mathur, R., 2009. Protective effects of propolis on inorganic mercury-induced oxidative stress in mice. Indian Journal of Experimental Biology, 47:264–266.

Downloads

Published

30-09-2025

Data Availability Statement

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Issue

Section

Research Articles

How to Cite

Tunç, A., Kaya, E., & Birdane, F. (2025). Effect of propolis supplementation on haematological and biochemical parameters in lambs. South African Journal of Animal Science, 55(9), 456–466. https://doi.org/10.17159/sajas.v55i9.03

Similar Articles

1-10 of 17

You may also start an advanced similarity search for this article.