Effects of different feeding regimes on growth performance, intake, digestibility, and ruminal metabolic and morphological characteristics in Awassi lambs

Authors

DOI:

https://doi.org/10.17159/sajas.v55i12.01

Keywords:

bacterial activity, fibre source, animal production, rumen fermentation, sheep

Abstract

The aim of this research was to investigate the effects of different feeding regimes on the growth performance, nutrient intake, apparent digestibility, and ruminal metabolic and morphological characteristics of Awassi lambs. A total of 96 lambs (initial age: 10 ± 1 weeks; mean body weight: 23.62 ± 0.01 kg) were divided into four dietary groups in a completely randomised design (eight replicates/diet, three lambs/replicate). The four diets were as follows: control (CON: 50% barley grain, 50% long lucerne/alfalfa hay), diet 1 (D1: 100% complete pelleted feed), diet 2 (D2: 40% concentrate pellets, 60% long lucerne hay), and diet 3 (D3: 60% concentrate pellets, 40% roughage pellets). The lambs were fed the four diets for 84 days, and growth performance indicators were assessed twice (every 42 days) during this period. After 84 days, all target parameters, including morphometry, fermentation indicators, and bacterial activity in the lambs' rumens, were assessed. The primary findings indicated that treatments D2 and D3 led to increased body weights, weight gains, and feed intakes. Lambs fed D2 had a higher dry matter intake and nutrient digestibility, along with increased papilla height, density, and total surface area. Specific fibre-degrading bacteria were more prevalent in D1, D2, and D3 than in the CON lambs. The higher total volatile fatty acid concentrations measured in the rumens of the D1, D2, and D3 lambs signified an improved fermentation profile. In conclusion, these results highlight the potential of complete diets and concentrate pellets with roughage sources to optimise performance, rumen health, and nutrient utilisation in lambs.

(Submitted 31 August 2025; Accepted 21 November 2025; Published 03 December 2025)

References

Adejoro, F.A., Hassen, A., Akanmu, A.M., & Morgavi, D.P., 2020. Replacing urea with nitrate as a non-protein nitrogen source increases lambs' growth and reduces methane production, whereas acacia tannin has no effect. Animal Feed Science and Technology, 259:114360.‏ DOI: https://doi.org/10.1016/j.anifeedsci.2019.114360

Aguayo-Ulloa, L.A., Miranda-De, L., Lama, G.C., Pascual-Alonso, M., Fuchs, K., Olleta, J.L., Campo, M.M., & María, G.A., 2013. Effect of feeding regime during finishing on lamb welfare, production performance and meat quality. Small Ruminant Research, 111(1–3):147–156.‏ DOI: https://doi.org/10.1016/j.smallrumres.2012.09.011

Alhidary, I.A., Abdelrahman, M.M., & Khan, R.U., 2016. Comparative effects of direct-fed microbial alone or with a traces mineral supplement on the productive performance, blood metabolites and antioxidant status of grazing Awassi lambs. Environmental Science and Pollution Research, 23:25218–25223. DOI: https://doi.org/10.1007/s11356-016-7684-z

Alshamiry, F.A., Alharthi, A.S., Al-Baadani, H.H., Aljumaah, R.S., & Alhidary, I.A., 2023. Growth rates, carcass traits, meat yield, and fatty acid composition in growing lambs under different feeding regimes. Life, 13(2):409.‏ DOI: https://doi.org/10.3390/life13020409

Andrés, S., Jaramillo, E., Bodas, R., Blanco, C., Benavides, J., Fernández, P., & Giráldez, F.J., 2018. Grain grinding size of cereals in complete pelleted diets for growing lambs: Effects on ruminal microbiota and fermentation. Small Ruminant Research, 159:38–44.‏ DOI: https://doi.org/10.1016/j.smallrumres.2017.12.009

AOAC, 2019. Official Methods of Analysis of AOAC International (21st edition), volume 1. Ed: Latimer, G.W., AOAC International, Gaithersburg, Maryland, USA.

Arjmand, M., Kiani, A., Azizi, A., Fadayifar, A., Azarfar, A., & Ponnampalam, E.N., 2022. Effects of dietary concentrate level and feeding length on nutrient digestibility, rumen hydrolytic enzymes activity, intermediary metabolites, and feeding behavior in growing fat-tailed lambs: Iranian feedlot system. Small Ruminant Research, 217:106832.‏ DOI: https://doi.org/10.1016/j.smallrumres.2022.106832

‏Ata, M., 2016. Effect of hydroponic barley fodder on Awassi lamb’s performance. Journal of Biology, Agriculture and Healthcare, 6:60–64.

Baldi, G., Chauhan, S.S., Linden, N., Dunshea, F.R., Hopkins, D.L., Rossi, C.S., & Ponnampalam, E.N., 2019. Comparison of a grain-based diet supplemented with synthetic vitamin E versus a lucerne (alfalfa) hay-based diet fed to lambs in terms of carcass traits, muscle vitamin E, fatty acid content, lipid oxidation, and retail colour of meat. Meat Science, 148:105–112.‏ DOI: https://doi.org/10.1016/j.meatsci.2018.10.013

Beigh, Y.A., Ganai, A.M., & Ahmad, H.A., 2017. Prospects of complete feed system in ruminant feeding: A review. Veterinary World, 10(4):424.‏ DOI: https://doi.org/10.14202/vetworld.2017.424-437

Böck, M.J., Simões, R.R., Rici, R.E.G., de Carvalho, S., do Santos Roberts, J.V., & de Morais‐Pinto, L., 2023. Morphological aspects of rumen papillae of lambs fed agroindustrial wastes. Animal Science Journal, 94(1):13897.‏ DOI: https://doi.org/10.1111/asj.13897

Broderick, G.A. & Kang, J.H., 1980. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science, 63(1):64–75.‏ DOI: https://doi.org/10.3168/jds.S0022-0302(80)82888-8

Chen, H., Wang, C., Huasai, S., & Chen, A., 2021. Effects of dietary forage to concentrate ratio on nutrient digestibility, ruminal fermentation and rumen bacterial composition in Angus cows. Scientific Reports, 11(1):17023. DOI: https://doi.org/10.1038/s41598-021-96580-5

Chen, Y., Gong, X., Yang, T., Jiang, M., Wang, L., Zhan, K., & Zhao, G., 2021. Ginkgo biloba L. residues partially replacing alfalfa hay pellet in pelleted total mixed ration on growth performance, serum biochemical parameters, rumen fermentation, immune function and meat quality in finishing Haimen White goats. Animals, 11(11):3046.‏ DOI: https://doi.org/10.3390/ani11113046

Claffey, N.A., Fahey, A.G., Gkarane, V., Moloney, A.P., Monahan, F.J., & Diskin, M.G., 2018. Effect of forage to concentrate ratio and duration of feeding on growth and feed conversion efficiency of male lambs. Translational Animal Science, 2(4):419–427.‏ DOI: https://doi.org/10.1093/tas/txy071

Costa, E.D.S., Ribiero, C.V.D.M., Silva, T.M., Ribeiro, R.D.X., Vieira, J.F., Lima, A.D.O., & Oliveira, R.L., 2021. Intake, nutrient digestibility, nitrogen balance, serum metabolites and growth performance of lambs supplemented with Acacia mearnsii condensed tannin extract. Animal Feed Science and Technology, 272:114744.‏ DOI: https://doi.org/10.1016/j.anifeedsci.2020.114744

Costa, M., Alves, S.P., Francisco, A., Almeida, J., Alfaia, C.M., Martins, S.V., & Bessa, R.J.B., 2017. The reduction of starch in finishing diets supplemented with oil does not prevent the accumulation of trans-10 18:1 in lamb meat. Journal of Animal Science, 95(8):3745–3761. DOI: https://doi.org/10.2527/jas.2017.1578

Cui, K., Qi, M., Wang, S., Diao, Q., & Zhang, N., 2019. Dietary energy and protein levels influenced the growth performance, ruminal morphology and fermentation and microbial diversity of lambs. Scientific Reports, 9(1):16612. DOI: https://doi.org/10.1038/s41598-019-53279-y

Cunha, I.S., Barreto, C.C., Costa, O.Y., Bomfim, M.A., Castro, A.P., Kruger, R.H., & Quirino, B.F., 2011. Bacteria and Archaea community structure in the rumen microbiome of goats (Capra hircus) from the semiarid region of Brazil. Anaerobe, 17(3):118–124. DOI: https://doi.org/10.1016/j.anaerobe.2011.04.018

Delgadillo-Ruiz, L., Bañuelos-Valenzuela, R., GallegosFlores, P., Meza-López, C., Echavarría-Cháirez, F., & Val-ladares-Carranza, B., 2022. Ruminal bacteria and protozoa present in sheep supplemented with probiotics identified by counting and PCR endpoint. Abanico Veterinario, 12:1–18.‏ DOI: https://doi.org/10.21929/abavet2022.6

Dewhurst, R.J. & Newbold, J.R., 2022. Effect of ammonia concentration on rumen microbial protein production in vitro. British Journal of Nutrition, 127(6):847–849. DOI: https://doi.org/10.1017/S000711452100458X

El-Nomeary, Y.A., Abd El-Rahman, H.H., Shoukry, M.M., Abedo, A.A., Salman, F.M., & Mohamed, M.I., 2021. Effect of different dietary protein sources on digestibility and growth performance parameters in lambs. Bulletin of the National Research Centre, 45:1–11.‏ DOI: https://doi.org/10.1186/s42269-021-00486-1

Francisco, A., Alves, S.P., Portugal, P.V., Dentinho, M.T., Jerónimo, E., Sengo, S., & Santos-Silva, J., 2018. Effects of dietary inclusion of citrus pulp and rockrose soft stems and leaves on lamb meat quality and fatty acid composition. Animal, 12(4):872–881. DOI: https://doi.org/10.1017/S1751731117002269

Henderson, G., Cox, F., Ganesh, S., Jonker, A., Young, W., & Janssen, P.H., 2015. Rumen microbial community composition varies with diet and host, but a core microbiome is found across a wide geographical range. Scientific Reports, 5(1):14567.‏ DOI: https://doi.org/10.1038/srep14567

Herath, H.M., Pain, S.J., Kenyon, P.R., Blair, H.T., & Morel, P.C., 2021. Growth and body composition of artificially-reared lambs exposed to three different rearing regimens. Animals, 11(12):3370. DOI: https://doi.org/10.3390/ani11123370

Hua, W., Hai, J., & Wang, Q., 2011. Ruminal fermentation: A review of CNCPS research. Animal Husbandry and Feed Science, 32:31–33.

Ishaq, S.L., Lachman, M.M., Wenner, B.A., Baeza, A., Butler, M., Gates, E., & Yeoman, C.J., 2019. Pelleted-hay alfalfa feed increases sheep wether weight gain and rumen bacterial richness over loose-hay alfalfa feed. PloS One, 14(6):0215797.‏ DOI: https://doi.org/10.1371/journal.pone.0215797

Karimizadeh, E., Chaji, M., & Mohammadabadi, T., 2017. Effects of physical form of diet on nutrient digestibility, rumen fermentation, rumination, growth performance and protozoa population of finishing lambs. Animal Nutrition, 3(2):139–144.‏ DOI: https://doi.org/10.1016/j.aninu.2017.01.004

Khateri, N., Azizi, O., & Jahani-Azizabadi, H., 2017. Effects of a specific blend of essential oils on apparent nutrient digestion, rumen fermentation and rumen microbial populations in sheep fed a 50:50 alfalfa hay:concentrate diet. Asian-Australasian Journal of Animal Sciences, 30(3):370–378.‏ DOI: https://doi.org/10.5713/ajas.15.0865

Khurshid, M.A., Rashid, M.A., Yousaf, M.S., Naveed, S., Shahid, M.Q., & Rehman, H.U., 2023. Effect of straw particle size in high grain complete pelleted diet on growth performance, rumen pH, feeding behavior, nutrient digestibility, blood and carcass indices of fattening male goats. Small Ruminant Research, 219:106907. DOI: https://doi.org/10.1016/j.smallrumres.2023.106907

Lafi, S.Q., Talafha, A.Q., Giadinis, N., Kalaitzakis, E., Pourliotis, K., & Panousis, N., 2009. Factors affecting the reproductive performance of Awassi sheep flocks in north-east of Jordan: An epidemiological study. Tropical Animal Health and Production, 41(8):1755–1764.‏ DOI: https://doi.org/10.1007/s11250-009-9374-z

Li, B., Sun, X., Huo, Q., Zhang, G., Wu, T., You, P., & Song, B., 2021. Pelleting of a total mixed ration affects growth performance of fattening lambs. Frontiers in Veterinary Science, 8:629016.‏ DOI: https://doi.org/10.3389/fvets.2021.629016

Li, C., Zhang, Q., Wang, G., Niu, X., Wang, W., Li, F., & Zhang, Z., 2022. The functional development of the rumen is influenced by weaning and associated with ruminal microbiota in lambs. Animal Biotechnology, 33(4):612–628.‏ DOI: https://doi.org/10.1080/10495398.2020.1812618

Li, R., Teng, Z., Lang, C., Zhou, H., Zhong, W., Ban, Z., & Lou, Y., 2019. Effect of different forage-to-concentrate ratios on ruminal bacterial structure and real-time methane production in sheep. PloS One, 14(5):0214777. DOI: https://doi.org/10.1371/journal.pone.0214777

Li, Z., Wang, X., Wang, W., An, R., Wang, Y., Ren, Q., & Xuan, J., 2023. Benefits of tributyrin on growth performance, gastrointestinal tract development, ruminal bacteria and volatile fatty acid formation of weaned Small-Tailed Han lambs. Animal Nutrition, 15:187–196.‏ DOI: https://doi.org/10.1016/j.aninu.2023.08.006

Liu, S., Shah, A.M., Yuan, M., Kang, K., Wang, Z., Wang, L., & Peng, Q., 2022. Effects of dry yeast supplementation on growth performance, rumen fermentation characteristics, slaughter performance and microbial communities in beef cattle. Animal Biotechnology, 33(6):1150–1160.‏ DOI: https://doi.org/10.1080/10495398.2021.1878204

Mahboubi, A., Agnihotri, S., Uwineza, C., Jomnonkhaow, U., & Taherzadeh, M.J., 2022. Chapter 18 – Waste-derived volatile fatty acids for sustainable ruminant feed supplementation. In: Biomass, Biofuels, Biochemicals. Eds: Varjani, S., Pandey, A., Taherzadeh, M.J., Ngo, H.H., & Tyagi, R.D., Elsevier. pp. 407–430. DOI: https://doi.org/10.1016/B978-0-323-88511-9.00015-X

Mirzaei-Alamouti, H., Abdollahi, A., Rahimi, H., Moradi, S., Vazirigohar, M., & Aschenbach, J.R., 2021. Effects of dietary oil sources (sunflower and fish) on fermentation characteristics, epithelial gene expression and microbial community in the rumen of lambs fed a high-concentrate diet. Archives of Animal Nutrition, 75(6):405–421.‏ DOI: https://doi.org/10.1080/1745039X.2021.1997539

Mousa, G.A., Allak, M.A., Shehata, M.G., Hashem, N.M., & Hassan, O.G., 2022. Dietary supplementation with a combination of fibrolytic enzymes and probiotics improves digestibility, growth performance, blood metabolites, and economics of fattening lambs. Animals, 12(4):476.‏ DOI: https://doi.org/10.3390/ani12040476

Na, S.W., 2022. Understanding the role of rumen epithelial host-microbe interactions in cattle feed efficiency. Animal Nutrition, 10:41–53. DOI: https://doi.org/10.1016/j.aninu.2022.04.002

Nagi, P.R.S., Reddy, D.N., Nagalakshmi, D., Reddy, Y.R., & Raghunandan, T., 2012. Effect of particle size of paddy straw on physical characteristics and performance of lambs fed paddy straw based complete diets. Animal Nutrition and Feed Technology, 12:111–119.

Neto, A.B., Herbster, C.J.L., Geraseev, L.C., Junior, G.M., Nascimento, D.R., Rocha, A.C., & Pereira, E.S., 2023. Feed energy utilization by hair sheep: does the 0.82 conversion factor of digestible to metabolizable energy need to be revised? The Journal of Agricultural Science, 161(5):734–742. DOI: https://doi.org/10.1017/S0021859623000515

National Research Council (NRC), 2007. Nutrient requirements of small ruminants (1st edition). National Academy Press, Washington, DC, USA.

Ojo, V.O.A., Oyaniran, D.K., Ogunsakin, A.O., Aderinboye, R.Y., Adelusi, O.O., & Odusoga, F.S., 2019. Effects of supplementing herbaceous forage legume pellets on growth indices and blood profile of West African dwarf sheep fed Guinea grass. Tropical Animal Health and Production, 51(4):867–877.‏ DOI: https://doi.org/10.1007/s11250-018-1767-4

Pazoki, A., Ghorbani, G.R., Kargar, S., Sadeghi-Sefidmazgi, A., Drackley, J.K., & Ghaffari, M.H., 2017. Growth performance, nutrient digestibility, ruminal fermentation, and rumen development of calves during transition from liquid to solid feed: Effects of physical form of starter feed and forage provision. Animal Feed Science and Technology, 234:173–185.‏ DOI: https://doi.org/10.1016/j.anifeedsci.2017.06.004

Pelegrin-Valls, J., Serrano-Pérez, B., Villalba, D., Martín-Alonso, M.J., Bertolín, J.R., Joy, M., & Álvarez-Rodríguez, J., 2020. Effect of dietary crude protein on productive efficiency, nutrient digestibility, blood metabolites and gastrointestinal immune markers in light lambs. Animals, 10(2):328.‏ DOI: https://doi.org/10.3390/ani10020328

Pereira, T.L., Fernandes, A.R.M., Oliveira, E.R., Cônsolo, N.R.B., Marques, O.F.C., Maciel, T.P., & Gandra, J.R., 2020. Serum metabolomic fingerprints of lambs fed chitosan and its association with performance and meat quality traits. Animal, 14:1987–1998. DOI: https://doi.org/10.1017/S1751731120000749

Prathap, P., Chauhan, S.S., Leury, B.J., Cottrell, J.J., Joy, A., Zhang, M., & Dunshea, F.R., 2023. Effects of feeding a commercial starch binding agent during heat stress on enteric methane emission, rumen volatile fatty acid contents, and diet digestibility of merino lambs. Atmosphere, 14(3):605.‏ DOI: https://doi.org/10.3390/atmos14030605

Retnani, Y., Risyahadi, S.T., Qomariyah, N., Barkah, N.N., Taryati, T., & Jayanegara, A., 2022. Comparison between pelleted and unpelleted feed forms on the performance and digestion of small ruminants: a meta-analysis. Journal of Animal and Feed Sciences, 31(2):97–108. DOI: https://doi.org/10.22358/jafs/149192/2022

SAS Institute, 2008. SAS Users Guide: Statistics. SAS Institute Inc., Cary, NC, USA.

Sohail, M.A., Rashid, M.A., Habib, H.F., Malik, M.I., Yousaf, M.S., & Rehman, H., 2022. Effects of physical form and wheat straw level in the diet on growth performance, nutrient digestibility, rumen papillae morphometry, and carcass characteristics in Lohi lambs. Animal Production Science, 62(18):1805–1815. DOI: https://doi.org/10.1071/AN21559

Soltani, E., Naserian, A.A., Khan, M.A., Ghaffari, M.H., & Malekkhahi, M., 2020. Effects of conditioner retention time during pelleting of starter feed on nutrient digestibility, ruminal fermentation, blood metabolites, and performance of Holstein female dairy calves. Journal of Dairy Science, 103:8910–8921. DOI: https://doi.org/10.3168/jds.2020-18345

Tian, G., Zhang, X., Hao, X., & Zhang, J., 2023. Effects of curcumin on growth performance, ruminal fermentation, rumen microbial protein synthesis, and serum antioxidant capacity in housed growing lambs. Animals, 13(9):1439.‏ DOI: https://doi.org/10.3390/ani13091439

Tripathi, M.K., Chaturvedi, O.H., Karim, S.A., Singh, V.K., & Sisodiya, S.L., 2007. Effect of different levels of concentrate allowances on rumen fluid pH, nutrient digestion, nitrogen retention and growth performance of weaner lambs. Small Ruminant Research, 72(2–3):178–186.‏ DOI: https://doi.org/10.1016/j.smallrumres.2006.10.008

Trottier, K., 2020. Determining the ideal forage-to-concentrate ratio for finishing lambs. PhD thesis, University of Guelph, Canada.

Van Soest, P.J., Robertson, J.B., & Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10):3583–3597. DOI: https://doi.org/10.3168/jds.S0022-0302(91)78551-2

Wang, L., Qi, W., Mao, S., Zhu, W., & Liu, J., 2022. Effects of whole corn high-grain diet feeding on ruminal bacterial community and epithelial gene expression related to VFA absorption and metabolism in fattening lambs. Journal of Animal Science, 100(3):56.‏ DOI: https://doi.org/10.1093/jas/skac056

Wang, Q., Zeng, Y., Zeng, X., Wang, X., Wang, Y., Dai, C., & Yang, H., 2021. Effects of dietary energy levels on rumen fermentation, gastrointestinal tract histology, and bacterial community diversity in fattening male Hu lambs. Frontiers in Microbiology, 12:695445.‏ DOI: https://doi.org/10.3389/fmicb.2021.695445

Wang, R., He, S., Huang, D., Wu, D., Peng, H., He, S., & Li, Q., 2023. The response of rumen pH, fermentation parameters and rumen bacteria to feeds of different concentrate to roughage ratios in buffalos. Frontiers in Microbiomes, 1:1053794.‏ DOI: https://doi.org/10.3389/frmbi.2022.1053794

Wang, W., Patra, A.K., Puchala, R., Ribeiro, L., Gipson, T.A., & Goetsch, A. L., 2022. Effects of dietary inclusion of tannin-rich Sericea lespedeza hay on relationships among linear body measurements, body condition score, body mass indexes, and performance of growing Alpine doelings and Katahdin ewe lambs. Animals, 12(22):3183.‏ DOI: https://doi.org/10.3390/ani12223183

Wu, Q.C., Wang, W.K., Zhang, F., Li, W.J., Wang, Y.L., Lv, L.K., & Yang, H.J., 2022. Dietary cysteamine supplementation remarkably increased feed efficiency and shifted rumen fermentation toward glucogenic propionate production via enrichment of Prevotella in feedlot lambs. Microorganisms, 10(6):1105.‏ DOI: https://doi.org/10.3390/microorganisms10061105

Yang, Y., Wang, B., Li, H., Chen, B.Y., & Yu, Z., 2022. Effects of pelletized corn straw and alfalfa hay-based total mixed ration on growth performance, blood characteristics and rumen fermentation of small-tailed Han sheep. Animal Nutrition and Feed Technology, 22(3):469–480.

Downloads

Published

03-12-2025

Data Availability Statement

The data and analyses presented in this paper are freely available from the corresponding author (H.H. Al-Baadani).

 

Issue

Section

Research Articles

How to Cite

Aboragah, A., Al-Baadani, H., Alharthi, A., Almaarik, B., & Alhidary, I. (2025). Effects of different feeding regimes on growth performance, intake, digestibility, and ruminal metabolic and morphological characteristics in Awassi lambs. South African Journal of Animal Science, 55(12), 561–576. https://doi.org/10.17159/sajas.v55i12.01
Views
  • Abstract 320
  • PDF 123

Similar Articles

1-10 of 43

You may also start an advanced similarity search for this article.